两种开封产黄色菊花的体外抗氧化活性

采用清除DPPH自由基、ABTS自由基和铁离子还原/抗氧化能力(tRAP)方法,评价开封产黄色菊花(春日剑山和麦浪)的体外总抗氧化活性,并将所测结果与水溶性维生素E(6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylicacid,Trolox)及阳性对照二丁基羟基甲苯(BHT)进行比较.结果发现,不同菊花的不同溶剂提取物抗氧化活性不同.随着提取溶刺极性的增大,同种菊花不同溶剂提取物总的抗氧化活性逐渐增大.即甲醇提取物)乙酸乙酯提取物)石油醚提取物.所有提取物中,麦浪甲醇提取物的抗氧化活性最好.它清除DPPH自由基的能力(IC_(50)=20.01mg/L)略低于BHT(IC_(50)=18.92mg/L),清除ABTS自由基的能力(IC_(50)=25.93mg/L)约为BHT(IC_(50)值为7.72mg/L)的1/3.

Antioxidant activity of extracts from two species of yellow chrysanthemum morifolium Ramat in Kaifeng in vitro

The total antioxidant activity of two species Chrysanthemum morifolium Ramat in Kaifeng in vitro, including Chunrijianshan and Mailang,was assayed by three methods, namely, DPPH free radical scavenging, ABTS free radical scavenging, and FRAP assay. Moreover, the results were compared with 6 - hydroxy - 2, 5, 7, 8 - tetramethylchroman-2- carboxylic acid (Trolox) and positive control 2,6- Di- tert- butyl-4- methylphenol ( BHT).The results showed that different extracts had different activity. In the same species of C. morifolium, the methanol extract generally showed higher total antioxidant activity than that of the ethyl acetate extract, and the petroleum ether extracts hardly had antioxidant activity.Among the six extracts, Mailang exhibited the highest total antioxidant activity.It showed slightly lower DPPH radical scavenging activity with IC_(50) value of 20.01 mg/L than that of BHT with IC_(50)value of 18.92mg/L, and showed ABTS radical scavenging activity with IC_(50) value of 25.93mg/L, about one third of BHT with IC_(50) value of 7.72mg/L.