毛细管电泳电化学检测水果中的抗坏血酸

利用毛细管电泳电化学检测法检测了抗坏血酸,考察了缓冲液的pH值、缓冲液浓度、分离电压、检测电势等实验参数对检测的影响。在最佳实验条件下,检测抗坏血酸的浓度范围是(1.0×10-5-5.0×10-3)mol.L-1,线性回归系数为0.9995,浓度检测限(S/N=3)为3.7×10-6mol.L-1,并以此方法检测了新鲜水果中的抗坏血酸的含量。

Determination of Ascorbic Acid in Pomelo by Capillary Electrophoresis with Electrochemical Detection

Ascorbic acid was detected by capillary electrophoresis with electrochemical detection. The effects of the running buffer pH, running buffer concentration, separation voltage and detected potential were studied. Under the optimum conditions of detection, the linear range of ascorbic acid was（1.0×10^-5.0×10^-3）mol·L^-1. The correlation coefficient of the method was 0.9995. The limit of detection （ S/N=3 ） was 3.7×10^-6mol·L-1. The method was used to detect ascorbic acid in fresh fruit.