Conversion of natural cellulosic substrates into fermentable sugar by recombinant fungi strains |
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Authors: | M K Tahoun and Aliaa A Ibrahim |
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Affiliation: | (1) Radiation Biotechnology Unit, Division of Radiation Biology and Radiation Protection, Institute of Nuclear Medicine and Allied Sciences, New Delhi, 110054, India;(2) Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA |
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Abstract: | The use of intergeneric recombinant fungi strains for the degradation of natural cellulosic substrates gave rise to various degrees of hydrolysis rates. Pretreating crude cellulose with alkaline or dilute acidic catalysts and heating removed the lignocellulosic fraction that is not attacked by microbial cellulase system, resulting in higher activity values and hydrolysis rates than when untreated substrates were used. Pretreated wheat straw was the best substrate, giving higher hydrolysis rates expressed as glucose ml–1 min–1 by recombinant strain 5–3-1 that exeeded other fungi strains investigated. |
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