胞内pH法评价酵母活性的研究

酵母细胞内pH与酵母生长和发酵状况密切相关，用胞内质子的释放能力可以衡量酵母细胞的生理状态。为了测定质子在酵母生理条件下的释放能力．在较低pH下，利用荧光法来测量胞内pH，简称ICP法。通过发酵试验证明，ICP法对于准确地评估啤酒生产过程中酵母细胞活性的细微区别，是非常有效的。

Method for Evaluation of Yeast Vitality by Measuring Intracellular pH

The intracellular pH of yeast plays an very important role during the yeast growth and fermentation, and the proton pump activity can be taken to measure the yeast physiological conditions. In order to analysis proton extrusion from brewer's yeast under physiological conditions, the method of measuring intracellular pH was adopted which was named ICP method for short. Investigations including fermentation experiments were carried out. As a result, the ICP method turned out to be very effective to assess the subtle differences of vitality of yeast which happens in the practical brewing conditions.