地蚕中低聚糖的提取及其诱导植物抗病性的研究

采用多级水提醇沉法来提取地蚕中低聚糖,通过实验确定了从地蚕中提取低聚糖的工艺条件:料液比为10∶1,80℃下熔煮2h,提取率可达到80%。采用不同浓度梯度的乙醇进行多级沉淀得到不同成分的低聚糖,并用提取的低聚糖喷雾处理小麦植株,诱导小麦对条锈病的抗病性,实验结果表明,用90%乙醇提取的低聚糖处理后病指下降百分率最高,平均可达80%以上。

Extraction of Oligosaccharides from Stachys geobombycis and It is Application on the Disease Resistance of Plant

Oligosaccharide was extracted from Stachys geobombycis with hot water and alcohol. The Stacgts geobombycis wasboiled at 80℃ for 2.5 h with liquid-to-solid ratio of 10:1 ml/g and the content of total polysaccharide in supernatant fluid could reach80% after filtration. Alcohol in different concentration was used to treat the filtrate liquor through a fractionated precipitationway to get the different oligosaccharide, which was then used to treat wheat to induce the resistance to wheat stripe rust.Results show that the alcohol in higher concentration could get the oligosaccharide, which is more helpful to induce the resis-tance of wheat to stripe rust.