水产品中微量甲醛的色差法快速检测

为在水产品物流现场快速定量检测微量甲醛,利用色差仪对甲醛与间苯三酚在碱性条件下反应生成的红色过渡物质进行明度和色度的测定,并计算色差值,从而测得样品中微量甲醛的浓度.考察了间苯三酚浓度、氢氧化钠浓度和显色剂用量的选择以获得最佳检测条件,并通过精密度实验、干扰性实验和回收率实验评价该方法.结果表明,反应体系采用质量分数0.01%间苯三酚、质量分数2%氢氧化钠、被测液与显色剂用量的体积比为2:1的检测效果最佳;该方法使用试剂少、显色快、抗干扰性强、检测限可达0.1 mg/kg、回收率达96%～102%.因此,该方法操作简单、结果可靠、仪器携带方便,不需现场加热和取电,适用于水产品物流现场微量甲醛的快速定量检测.

Rapid Determination of Trace Amounts of Formaldehyde Based on Chromatic Aberration in Aquatic Product Logistics

With the target to develop a rapidly and quantified assay method for trace amounts of formaldehyde in aquatic product logistics, the composition of reaction system, like the concentration of phloroglucinol, sodium hydroxide, and colorimetric reagent were optimized and described as follows: 0. 01% phloroglucinol,2% sodium hydroxide (2%) under the volume ratio (measured liquid: colorimetric reagent) condition of 2 : 1. With the optimum conditions, the determination limit and the recovery rate can reach at 0.1 mg/mL and 96% ～ 102%, respectively.