| 低温纤维素降解菌的筛选及其产酶研究 |
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| 引用本文： | 任红梅, 刘左军, 袁惠君, 水清照, 禄亚芬. 低温纤维素降解菌的筛选及其产酶研究[J]. 食品工业科技, 2013, (19): 127-130. DOI: 10.13386/j.issn1002-0306.2013.19.040 |
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| 作者姓名： | 任红梅 刘左军 袁惠君 水清照 禄亚芬 |
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| 作者单位： | 1.兰州理工大学生命科学与工程学院 |
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| 摘 要： | 从甘南州玛曲县高寒草甸土壤中筛选出一株低温纤维素降解菌M7。通过形态观察、生理生化及16SrRNA序列比对分析,发现菌株M7与Bacillus subtili strain XS6进化距离最近,初步鉴定为枯草芽孢杆菌（Bacillus subtili）。通过Box-Benhnken中心组合设计和响应面分析法（RSM）,对其生长和产羧甲基纤维素酶（CMCase）条件进行优化。结果表明:温度20℃,培养基初始pH5.0,转速175r/min,酵母膏2.5g/L和微晶纤维素2.5g/L,蛋白胨5g/L,菌株M7所产CMCase达到最大值。
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| 关 键 词： | 羧甲基纤维素酶 响应面 低温细菌 高寒草甸 优化 |
| 收稿时间： | 2013-03-05 |
Study on screening and cellulase production of cold-adaped bacterium |
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| REN Hong-mei, LIU Zuo-jun, YUAN Hui-jun, SHUI Qing-zhao, LU Ya-fen. Study on screening and cellulase production of cold-adaped bacterium[J]. Science and Technology of Food Industry, 2013, (19): 127-130. DOI: 10.13386/j.issn1002-0306.2013.19.040 |
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| Authors: | REN Hong-mei LIU Zuo-jun YUAN Hui-jun SHUI Qing-zhao LU Ya-fen |
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| Affiliation: | 1.School of Life Science and Technology, Lanzhou University of Technology |
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| Abstract: | A strain M7 with cellulose degradation ability was screened from alpine meadow soil of Maqu county in Gansu province.It is identified as Bacillus subtilis by morphology, physiological and biochemical property and 16SrRNA sequence analysis.The initial study on its producing cellulase showed that the temperature was 20℃, initial pH5.0, revolving speed 175r/min, peptone 5g/L, yeast extract 2.5g/L and microcrystalline cellulose 2.5g/L.After the optimization of Box-Benhnken design and response surface methodology, the cellulase activity reached the maximum. |
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| Keywords: | carboxymethyl cellulose (CMCase) response surface methodology cold-adapted bacterium alpine meadow optimization |
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