大豆低聚肽抗氧化及抗疲劳作用评价

对大豆低聚肽的抗氧化活性进行了研究,分别测定大豆低聚肽的总抗氧化能力、抗超氧阴离子自由基能力、羟自由基和烷基自由基的清除率;并通过动物实验考察大豆低聚肽的抗疲劳作用,测定小鼠力竭游泳时间、血浆尿素氮含量、肝糖原和肌糖原含量。结果表明:大豆低聚肽总抗氧化能力最大为13.86U/mL,抗超氧阴离子自由基能力最大为158.67U/L,对羟自由基的最大清除率为17.11%,对烷基自由基的最大清除率为31.97%;对大豆低聚肽抗疲劳作用的研究表明,游泳时间最长为高剂量组(8.0g/kg)45.51min,血浆尿素氮含量最低为高剂量组(8.0g/kg)17.05mmol/L,肝糖原含量最高为高剂量组(8.0g/kg)17.45mg/g,肌糖原最高含量为中剂量组(4.0g/kg)1.53mg/g。由此可见,大豆低聚肽具有一定的抗氧化活性及抗疲劳作用。 

Evaluation of the antioxidant and anti-fatigue effect of soy oligopeptide

Total antioxidant capacity, superoxide anion radical scavenging, the clearance rate of hydroxyl and alkyl radical scavenging of soy oligopeptide was determined respectively in order to study the antioxidant activities of soybean oligopeptides.Swimming time, the content of plasma urea nitrogen, liver glycogen and muscle glycogen of mice was determined respectively through animal experiments in order to test the effect of soy oligopeptide anti-fatigue.Results showed that the total antioxidant ability was 13.86U/mL, the maximum ability to anti-superoxide anion was 158.67U/L, the maximum inhibition rate to hydroxy free radical was 17.11%, the maximum inhibition rate to alkyl free radical was 31.97%, the anti-fatigue effect of soybean oligopeptide studies showed that, the longest swimming time was 45.51min for the high dose group (8.0g/kg) , the lowest BUN content was 17.05mmol/L for the high dose group (8.0g/kg) , the highest content of liver glycogen was 17.45mg/g tissue for the high dose group (8.0g/kg) , the highest content of muscle glycogen was 1.53mg/g tissue for the medium dose group (4.0g/kg) .Thus, soybean oligopeptide had antioxidant and anti- fatigue effect.