转Bar、Bt基因双抗大米外源基因在食品加工过程中稳定性的研究

旨在了解转Bar、Bt基因双抗大米外源基因在酒酿和米果加工过程中的稳定性,以转Bar、Bt基因双抗大米为原料,进行了6种不同方式的加工,并针对其加工品中外源基因(包括CaMV35S启动子、Ubiquitin启动子、NOS终止子、Bar基因和Bt基因)进行不同大小片段的PCR扩增。结果表明,实验中长度小于200bp的片段均具有较高的GC含量(NOS终止子除外)和良好的稳定性。 

Study on stability of exogenous genes in transgenic rice with Bar and Bt gene during food processing

In order to assess the effect of food processing on the stability of exogenous genes in transgenic rice, fermented glutinous rice and rice cracker were made by using transgenic rice with Bar and Bt gene as main material. The degradation of exogenous gene (CaMV35S promoter, Ubiquitin promoter, NOS terminator, Bar gene and Bt gene) were detected by using different fragments-size PCR primers. It was found that the stability of genes was different under different processing conditions. The fragments whose length is under 200bp are characteristic of higher GC content (except NOS terminator) and good stability.