响应面法优化马血中SOD超声波提取工艺研究

以SOD比活力为响应值,在单因素实验的基础上,筛选出超声时间、超声功率、超声温度三个对提取影响较大的因素,利用响应面法进行优化,并验证优化方案。结果表明,SOD超声波提取的优化参数为:超声时间15min、超声功率220W、超声温度31℃。在此条件下,SOD比活力为77.5U/mg,与模型高度拟合。 

Optimization of ultrasonic extraction technique of SOD from horse blood by response surface methodology

Based on single factor experiment, three main factors were creened out, including the ultrasonic time, ultrasonic power, ultrasonic temperature. Optimization of these factors by using response surface methodology was studied. Results of response surface analysis showed that the optimum ultrasonic extraction conditions of SOD were as follows : ultrasonic time 15min , ultrasonic power 220W , the ultrasonic temperature 31 ℃ . Under these conditions, specific activity of SOD was 77.5U/mg and which was in good agreement with the predicted value.