响应面法优化藠头中皂苷的提取工艺研究

利用响应面法对超声提取藠头皂苷的工艺进行优化。实验研究了超声条件下影响藠头皂苷提取的几个因素,包括超声时间、乙醇浓度、料液比、超声温度,并通过响应面法优化提取工艺。根据中心组合设计原理采用三因子三水平的响应面分析法,通过对各因子显著性和交互作用的分析,得出了超声提取藠头皂苷的最佳工艺条件为:乙醇浓度71%,超声温度55℃、料液比1:16,此时藠头皂苷的得率为3.71%。说明响应面法能较好地对藠头皂苷的超声提取工艺进行回归分析和参数优化。 

Optmization of the extraction technology of the Allium chinensis G.Don saponins by response surface methodology

To optimize the extraction technique for saponins from Allium chinensis G.Don by supersonic wave, supersonic extracting time, and the ratio of sample to solvent, content of ethanol and supersonic extracting temperature were studied as the single factors.The best extraction method was found through the response surface method.Three levels of the factors (the ratio of sample to solvent, contentof ethanol and supersonic extracting temperature) were selected for the Box-Behnken factorial design to establish a quadric regression equation for describing the yield of saponins.By response surface analysis, the optimum extraction conditions were obtained as follows:content of ethanol 71%, extraction temperature 55℃ and the ratio of sample to solvent 1:16.Under such conditions the yield of total saponins could be up to 3.71%, which indicated that response surface analysis could be an effective method to optimize the extraction technology of the Allium chinensis G.Don saponins.