响应面法优化苦瓜籽蛋白质提取工艺

以苦瓜籽为原料,采用紫外光谱和响应面法研究盐溶法提取苦瓜籽蛋白质的最佳工艺。以苦瓜籽蛋白质的提取率为指标,考察NaCl浓度、pH、提取时间对苦瓜籽蛋白质提取率的影响。在单因素实验的基础上,通过Box-Benhnken中心组合设计和响应面分析法,确定苦瓜籽蛋白质的最佳提取工艺。结果表明:在料液比为1:6,提取温度为4℃下,NaCl浓度1.3mol/L,pH8.8,提取时间3.6h,苦瓜籽蛋白质的提取率为23.80%,较优化前提高了3.24倍,同时建立了盐提蛋白质的二次多项数学模型,对苦瓜籽蛋白质的提取具有很好的预测作用。此外,通过凯氏定氮法确定苦瓜籽中蛋白质含量为32.13%。 

Optimization of protein extraction from defatted bitter melon seed using response surface methodology

The optimum extracting conditions of protein from bitter melon seeds by the salt solvent method were studied.The effects of concentration of NaCl, pH and extraction time on the yield of protein were studied based on the extraction rate.According to the single factor experiments, the optimum extract process for bitter melon seed protein was determined through Box-Benhnken central composite design and response surface methodology.The results showed that the optimized extract process for bitter gourd seed protein was that the material/liquid ratio was 1:6 and the temperature was at 4℃, the concentration of NaCl was 1.3mol/L and pH8.8, at a extract time of 3.6h, the extraction rate of bitter gourd seed protein was 23.80%, 3.24 times higher than that before the optimization.In addition, a quadratic equation model for protein extraction was set up and it played an important role in predicting the extraction of protein.Furthermore, the protein content of bitter melon seeds was 32.13% determined by Kjeldahl method.