添加DHA的食用油对SD大鼠肝抗氧化活性的研究

为了研究添加DHA的食用油对肝抗氧化活性的影响,本文选取96只初断乳SD雄性大鼠(80±10)g,按照随机数字表法分为8组,阴性对照组(大豆油和花生油等比例混合)、阳性对照组(DHA藻油),以250、500、750 mg DHA分别调配入60 g大豆油、花生油中,作为DHA低、中、高剂量调配油的处理组。对各处理组按1 m L混合油/100 g体重·d喂养分别达到4周和8周时,对SD大鼠肝组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和乙酰胆碱酯酶(ACh E)活性进行检测。结果表明:DHA中剂量组大鼠肝组织SOD活性极显著高于阴性对照组(p<0.01),MDA含量极显著低于阴性对照组(p<0.01),ACh E活性无显著性差异(p>0.05);DHA高剂量组肝组织SOD活性显著低于阳性对照组(p<0.05),MDA含量显著高于阳性对照组(p<0.05),ACh E无显著性差异(p>0.05);其余各组ACh E、SOD活性及MDA含量均无显著性差异(p>0.05)。适量补充DHA,有增强大鼠机体抗氧化的能力,然而一旦DHA摄入过量,反而会对机体抗氧化能力产生消极作用。 

Research of the edible oil contained DHA affecting the antioxidant activity of SD rats livers

To study the effect of edible oil contained DHA on the antioxidant activity of liver,this paper selected96 early weaning SD male rats(80±10) g,which were divided into 8 groups based on the random number.Table the negative control group(mixing ratio of soybean and peanut oil),positive control group(DHA algae oil),250,500,750 mg DHA were seperately added to 60 g soybean oil,peanut oil,with low,medium,high dose DHA were treatment group.According to the dosage of 1 m L oil per 100 g weight a day and after feeding for 4weeks and 8 weeks respectively,the SOD activity,MDA content and ACh E in the liver tissues of SD rats were detected. The results showed that the SOD activity of liver tissue in middle dosage of DHA group was significantly higher than that in negative control group,the MDA content was significantly lower than that in positive control group and the ACh E had no significant difference. The SOD activity in high dose of DHA group was significantly lower and the MDA content was significantly higher than that in negative control group,positive control group,and the ACh E also had no significant difference. While the other groups had no significant difference in ACh E,SOD activity and MDA content. Appropriate supplement of DHA could enhance the rat body's antioxidant capacity,but the excessive DHA will bring a negative effect.