高通量快速检测母乳总蛋白、乳清蛋白和酪蛋白含量方法的比较研究

分别利用Lowry、BCA和Bradford三种方法对母乳中总蛋白、乳清蛋白和酪蛋白的含量进行了测定,并以凯氏定氮法为标准,对三种方法的检测结果进行了比较。结果表明Lowry和Bradford法适用于母乳中总蛋白含量的测定,Lowry法适于母乳中乳清蛋白含量的测定,Lowry、BCA和Bradford三种方法均适用于母乳中酪蛋白含量的测定。采用上述方法,只需要4 m L母乳样本,1.5 h内即可完成母乳总蛋白、乳清蛋白和酪蛋白的微量、高通量快速测定。上述方法适用于较大量母婴营养状况的调查研究,可为母乳化婴幼儿配方乳粉的研制以及促进婴幼儿生长发育提供科学依据。 

A comparative study of rapid and high- throughput detection method for total protein,whey protein and casein in human milk

The contents of human milk total protein,whey protein and casein were determined using Lowry,BCA and Bradford,respectively,and the results were compared with the Kjeldahl. The results showed that both Lowry and Bradford methods were suitable for determination of total protein content,while the whey protein content determined using Lowry method was closer to Kjeldahl. Lowry,BCA and Bradford methods were applicable for determination of the content of casein. Adopt the above- mentioned methods,the contents of human milk total protein,whey protein and casein can be rapidly determined within 1.5 h using only 4 m L human milk. These methods were suitable for the investigation of a large number of maternal and child nutritional status so that it provided the scientific basis for the development of human milk simulated infant formula and infant growth promotion.