青砖茶提取物不同极性部位对三种细菌的抑制作用研究

采用滤纸片法测定青砖茶提取物不同极性部位氯仿层、乙酸乙酯层、正丁醇层及水层对金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌的抑制性能,并测定它们的最小抑菌浓度和总酚含量。结果表明:乙酸乙酯层对三种菌的抑制效果最优,当其浓度为100 mg/m L时,对金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌的抑菌圈直径依次为29.19、27.97、27.35 mm;最小抑菌浓度依次为1.56、3.12、6.25 mg/m L;总酚含量高达81.62%,提示总酚为其抑菌的主要活性成分。 

Study on the inhibition of three bacterias by different polar fractions from qingzhuan brick tea extract

Agar diffusion method was used to investigate the inhibiting effect of Staphylococcus aureus,Escherichia coli and Bacillus subtilis by different polar fractions inculding chloroform fraction,ethyl acetate fraction,n- butyl alcohol fraction and water layer fraction from the Qingzhuan brick tea extract,and their minimum bacteriostatic concentration and total polyphenol content were determined. The results showed that the antimicrobial activity of ethyl acetate fraction among different polar fractions was the best and the antimicrobial diameters on inhibiting Staphylococcus aureus,Escherichia coliand Bacillus subtilisare were respectively 29.19,27.97,27.35 mm when its concentration was 100 mg / m L.The minimum inhibitory concentrations for Staphylococcus aureus,Escherichia coli and Bacillus subtilis were,3.12,6.25 mg / m L,respectively.The total polyphenol content in ethyl acetate fraction was as high as 81.62% and the total polyphenol may be the main active ingredients on its bacteriostasis.