新型中国被毛孢胞内多糖HSIPS2链构象及抗氧化活性研究

以发酵得到的中国被毛孢菌丝体为原料进行热水浸提,经过醇沉、脱蛋白、脱色素以及DEAE-Sepharose Fast Flow离子交换柱色谱和Sephacryl S-100凝胶柱色谱得到均一性多糖组分（HSIPS2）,并对该组分进行链构象及抗氧化活性分析。结果表明:HSIPS2的单糖组成及摩尔比例为葡萄糖∶半乳糖∶甘露糖∶核糖=48.52∶6.58∶5.17∶1.0,其绝对分子量为1.46×10⁴ g/mol,纯度为99.82%;该多糖在0.1 mol/L的Na NO3溶液中呈柔顺链构象,流体力学半径R_h=2.3 nm,M_L=334.05 nm^-1,q=0.70 nm,d=0.69 nm;同时,HSIPS2清除羟自由基的IC₅₀为0.749 mg/m L,氧自由基吸收能力（ORAC值）为872.80μmol TE/g,说明HSIPS2具有较高的抗氧化能力。 

Chain conformation and antioxidant activity of a novel intracellular polysaccharide HSIPS2 from Hirsutella sinensis

A novel intracellular polysaccharide,named HSIPS2,from Hirsutella sinensis was obtained by hot water extraction and alcohol precipitation,and then purified using DEAE-Sepharose Fast Flow chromatography and Sephacryl S-100 gel column chromatography. Its chain conformation and antioxidant activity were determined.The result showed that HSIPS2 was composed of glucose,galactose,mannose,and ribose with a molar ratio of48.52 ∶6.58 ∶5.17 ∶1.0. It was proved to be a novel lower dispersion polysaccharide with uniform molecular of1.463×10~4g/mol,and the purity was 99.82%. In 0.1 mol/L Na NO_3 aqueous solution,HSIPS2 existed as flexible chain conformation. The parameters of Rh,ML,q and d were 2.3 nm,334.05 nm~/(-1),0.70 nm,and 0.69 nm respectively.Moreover,the IC_(50) of its hydroxyl radical scavenging activity was identified to be 0.749 mg/m L and the value of ORAC was calculated to be 872.80 μmol TE/g. Hence,it was undoubtedly demonstrated that HSIPS2 exhibited potent antioxidant activity.