亚临界丙酮提取万寿菊渣中抗氧化活性成分的研究

研究了亚临界丙酮不同浓度、萃取温度、时间和液固比对万寿菊渣中黄酮、总酚等抗氧化活性物质提取效果的影响。结果表明,提取液中总黄酮、总酚含量基本上随丙酮浓度、萃取温度和液固比先增加后减少,最佳提取条件为丙酮浓度60%、温度140℃、提取时间30min,该条件下总黄酮得率为15.03%±0.04%,总酚得率为13.12%±0.07%,ABTS+·清除能力为（3.18±0.07）mmol TE/g,DPPH·清除能力为（3.42±0.12）mmol TE/g。通过与传统溶剂的振荡浸提法和亚临界水、亚临界乙醇提取方法的比较,亚临界丙酮提取具有明显优势。 

Subcritical acetone extraction of antioxidative components from marigold grounds

The subcritical acetone extraction method was used to extract antioxidative substances from marigold grounds. The effect of the concentration of subcritical acetone, extraction temperature, time and liquid to solid ratio on the extraction efficiency was investigated. The result showed that the content of total phenols and flavonoids of the marigold grounds extracts were generally increased with the elevating concentration of acetone, temperature, time and liquid to solid ratio at first, but the decreased instead. The best extraction condition of total phenols and flavonoids was 60% acetone, 140℃ and 30min. The yield of total flavonoids was15.03%±0.04%, the yield of total phenols was 13.12%±0.07%, the ABTS+· scavenging capacity was (3.18±0.07) mmol TE/g, the DPPH· scavenging capacity was (3.42 ±0.12) mmol TE/g. Compared with traditional solvent oscillation extraction and subcritical water, subcritical ethanol methods, subcritical acetone extraction had obvious advantages.