大孔吸附树脂纯化西兰花色素的研究

目的:筛选对西兰花色素具有良好选择性的大孔树脂,并研究其动态吸附与解析性能。方法:通过静态吸附特性研究,以吸附率和解析率为指标,从5种不同型号的大孔吸附树脂中筛选出对西兰花色素选择性好的树脂,再进而考察各种工艺参数对树脂吸附及洗脱的影响。结果:AB-8对西兰花色素具有良好的选择性,静态及动态吸附及解析特性研究显示其最佳的纯化工艺参数为:上样液浓度2.5mg/mL、流速1.0mL/min、pH4.0、温度50℃;洗脱液采用体积分数80%的乙醇、流速1.0mL/min。采用此工艺条件,西兰花色素色价从23.7提高到91.7。结论:AB-8型大孔树脂对西兰花色素具有良好的吸附与解析性能,适用于西兰花色素的纯化。 

Purification of pigment from Brassica oleracea L. var. botrytis L. by macroporous resin

Objective:To study the optimum purification conditions of pigment from Brassica oleracea L.var.botrytis L..Methods:Five kinds of macroporous resins were used to investigate the characteristics of static absorption.According to adsorption and deadsorption rate, a kind of macroporous resins was chose to investigate the characteristic of dynamic adsorption and deadsorption.Results:the AB-8 was the most suitable resin to purify pigment from B.oleracea.The optimal adsorption conditions were pH 4.0, concentration 2.5mg/ mL, temperature 50℃ and velocity 1.0mL/min.The optimal deadsorption conditions were 80% ethanol, velocity 1.0mL/min.The color value of pigment had been increased to 91.7 by this technological process, which nearly 3 times higher than that of pigment without purification (23.7) .Conclusion:AB-8 had good performance in purification of pigment from B.oleracea.The optimal process parameters provided basis for industrialization of this pigment.