| 海洋骨胶原低聚肽钙的分离纯化及结构鉴定 |
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| 引用本文： | 刘文颖, 任玮, 谷瑞增, 鲁军, 蔡木易. 海洋骨胶原低聚肽钙的分离纯化及结构鉴定[J]. 食品工业科技, 2016, (07): 49-51. DOI: 10.13386/j.issn1002-0306.2016.07.001 |
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| 作者姓名： | 刘文颖 任玮 谷瑞增 鲁军 蔡木易 |
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| 作者单位： | 1.中国食品发酵工业研究院北京市蛋白功能肽工程技术研究中心 |
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| 摘 要： | 以三文鱼骨作为原料,以碳酸钙作为钙源制备出海洋骨胶原低聚肽钙,对其总蛋白含量、螯合率、得率进行测定,结果表明总蛋白含量为62.56%±0.26%,螯合率为51.38%±0.09%,螯合物得率为42.06%±0.10%。然后利用反相高效液相色谱（RP-HPLC）对其进行分离纯化,收集得到8个组分峰,利用Q-TOF质谱仪测定肽序列,共分析出22个肽段,分子量大多在1000 u以下。
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| 关 键 词： | 海洋骨胶原低聚肽 肽钙螯合物 分离纯化 结构鉴定 |
| 收稿时间： | 2015-08-27 |
Separation,purification and structural identification of calcium- chelating Marine bone collagen oligopeptides |
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| LIU Wen- ying, REN Wei, GU Rui- zeng, LU Jun, CAI Mu-yi. Separation,purification and structural identification of calcium- chelating Marine bone collagen oligopeptides[J]. Science and Technology of Food Industry, 2016, (07): 49-51. DOI: 10.13386/j.issn1002-0306.2016.07.001 |
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| Authors: | LIU Wen- ying REN Wei GU Rui- zeng LU Jun CAI Mu-yi |
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| Affiliation: | 1.Beijing Engineering Research Center of Protein and Functional Peptides,China National Research Institute of Food and Fermentation Industries |
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| Abstract: | Calcium- chelating Marine bone collagen oligopeptides( MBCP- Ca) were prepared by using salmon bone as raw material and calcium carbonate as calcium resource.The total protein content,chelating rate and yield were determined. Results showed that the total protein content,chelating rate and yield were 62.56% ± 0.26%,51.38% ± 0.09% and 42.06% ± 0.10%,respectively.Then,MBCP- Ca was separated and purified by reversed phase high performance liquid chromatography( RP- HPLC).A total of 8 fractions were collected and subjected to Q- TOF mass spectrometer to identify peptide sequences. A total of 22 peptide sequences were identified,and most molecular weights of the peptides were below 1000 u. |
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| Keywords: | Marine bone collagen oligopeptides calcium-chelating peptides separation and purification structural identification |
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