PCR-DGGE技术及其在食品菌相分析中的应用

传统培养、鉴定的方法研究微生物菌相变化的缺点突出,不易对微生物的变化情况进行实时的分析。近年来,使用聚合酶链式反应-变性梯度凝胶电泳技术（PCR-DGGE）分析食品中菌群变化情况的优点逐渐突显。本文介绍了PCR-DGGE技术的原理、优点和缺点,以及在食品菌相研究中的应用,并对该技术在食品微生物研究中的前景进行了展望。 

PCR-DGGE technology and its application in analyzing food bacterial phase

The main shortages of traditional culture for identifying microbial phase in food are difficult to analysis dynamic changes of microorganisms at real time. In recent years, the advantages of polymerase chain reaction -denaturing gradient electrophoresis in revealing the changes of the microbial population are prominent. The principle, advantages and disadvantages of PCR-DGGE were introduced in this paper, the development and prospect of PCR-DGGE technology in food research and the microbial phase analysis were discussed either.