酶法制备沙漠果蛋白ACE抑制肽工艺的研究

在单酶筛选和单因素实验的基础上,采用响应面分析法优化沙漠果蛋白血管紧张素转换酶抑制肽(ACEIP)酶解制备的技术条件,主要研究了酶解时间、酶解温度、初始p H及底物浓度四个因素对酶解工艺条件的影响。以ACE抑制率为响应值,确定碱性蛋白酶的最佳酶解条件为:酶解时间114 min,酶解温度57℃,初始p H10,底物浓度3.24%,酶添加量2000 U/g,此时ACEIP抑制率为69.83%。本研究可为沙漠果蛋白的高效利用奠定基础。 

Preparation of ACE inhibitory peptides from Brazil nut protein by enzymatic hydrolysis

Based on single-enzyme screening and single-factor tests analysis,the preparation conditions of ACE inhibitory peptides from Brazil nut protein were studied with response surface analysis methodology. The effects of four factors,including the time of enzymatic hydrolysis,the temperature,initial p H and substrate concentration,were mainly discussed on the enzymatic hydrolysis. Using the ACE inhibitory rate as responses,the optimum conditions of alcalase enzymatic hydrolysis were determined as enzymatic hydrolysis time 114 min,enzymatic hydrolysis temperature 57 ℃,initial p H10,3.24% concentration of the substrate,dosage of enzyme2000 U/g,and then the inhibition ratio of ACEIP was 69.83%. The results of the research were the basis for the efficient utilization of the Brazil nut protein.