体外模拟消化过程中大豆蛋白的荧光光谱分析及热处理的影响

分析了不同温度热处理及不同时间热处理的大豆分离蛋白体外模拟消化过程产物的荧光光谱。结果表明:不同时间热处理及不同温度的热处理均对大豆蛋白的消化有一定促进作用,大豆蛋白的最佳热处理条件为85℃、20 min,蛋白质的消化程度最大。大豆分离蛋白经不同温度热处理后,消化1 h,消化产物的最大吸光波长(λ_max)即随着加热温度的上升而红移,在加热90℃时达到最大值后下降,而荧光强度呈现出先上升后下降的变化趋势。经过不同时间热处理后消化1 h,大豆分离蛋白消化产物的λ_max先上升后下降。且荧光强度随着加热时间的延长呈现出不同的变化趋势,在0²0 min不断升高时,20 min时达到最大值,而继续加热至60 min,荧光强度逐渐下降。 

Fluorescence spectra analysis of soybean protein in vitro under heat treatment

The characters of fluorescence spectra of soy protein isolate(SPI) solution in vitro of different heat treatment temperature and different heat treatment time. The results showed that heat treatment had a certain effect on the digestion of soybean protein at different heat treatment temperature and different heat treatment time. The best heat treatment condition of soybean protein was 85 ℃,20 min,which was the best degree and effectiveness of the vitro digestion. The soy protein isolate was heat-treated at different temperatures,digested1 h. With the rise of heating temperature,digested products of λ_max increasing redshift,then it started to decrease when the maximum value was reached at 90 ℃.And the fluorescence intensity increased with the heating time,and showed a trend of decline after the first rise. The in vitro simulated digestion of soybean protein isolate 1 h at different heating time,the isolated soy protein digested products of λ_max experienced a process,which was first increased,then decreased. And the fluorescence intensity with the increase of heating time and showed different trends.Growing within 0~20 min,reaching maximum at 20 min,continuing to heat up to 60 min,the fluorescence intensity decreased.