Efficacy of novel antimicrobials against clinical isolates of opportunistic amebas |
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Authors: | FL Schuster GS Visvesvara |
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Affiliation: | Department of Radiation Medicine, Georgetown University Medical Center, Washington, DC 20007, USA. |
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Abstract: | Using atomic force microscopy (AFM), we have investigated neutron-induced DNA double-strand breaks in plasmids in aqueous solution. AFM permits direct measurement of individual DNA molecules with an accuracy of a few nanometers. Furthermore, the analysis of the DNA fragment size distribution is non-parametric, whereas other methods are dependent on the model. Neutron irradiation of DNA results in the generation of many short fragments, an observation not made for damage induced by low-LET radiation. These data provide clear experimental evidence for the existence of clustered DNA double-strand breaks and demonstrate that short DNA fragments may be produced by such radiations in the absence of a nucleosomal DNA structure. |
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