基于转录组的氯吡脲对草莓果实成熟的调控机制研究

目的 通过对对照组和白熟期外源施用氯吡脲处理组的成熟草莓果实进行转录组学分析,探讨外源喷施氯吡脲影响草莓果实成熟的调控机制.方法 利用高通量测序技术RNA-Seq,结合生物信息学方法,进行差异基因的筛选和其通路的功能富集分析.结果 草莓白熟期喷施氯吡脲,成熟果实中有1176个基因表达上调,2031个基因表达下调.差异基...

Transcriptome-based to study the regulation mechanism of forchlorfenuron on strawberry fruit ripening

Objective To analyze the transcriptomics analysis of the mature strawberry fruits of the control group and the treatment group treated with exogenous forchlorfenuron during the white maturity period, and explore the regulatory mechanism of exogenous spraying of forchlorfenuron on strawberry fruit ripening. Methods The differential genes were screened and the functional enrichment of their pathways were analyzed by high-throughput sequencing technology RNA-Seq and bioinformatics methods. Results Totally 1176 genes were up-regulated and 2031 genes were down regulated in the mature strawberry fruits sprayed with chlorfenuron at white ripening stage. Enrichment analysis of differentially expressed genes revealed that plant pathogen interaction, plant hormone signaling, starch and sucrose metabolism, endoplasmic reticulum protein processing and secondary metabolite biosynthesis were the main metabolic pathways of differentially expressed genes. Conclusion Plant hormone signal transduction pathway, plant pathogen interaction pathway and secondary metabolite biosynthesis pathway are most significantly affected by chlorfenuron, and plant hormone signal transduction pathway affects strawberry growth and maturity through the joint regulation of different hormones. The results lays a foundation for the further research and mining of genes regulating the effect of chlorfenuron on strawberry fruit ripening.