COLOUR REACTION SCREENING OF CLONES WITH DIACETYL AND ACETOIN REDUCTASE ACTIVITIES |
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| Authors: | O Legeay R Ratomahenina C Chabalier P Galzy E Goessens |
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| Abstract: | A genomic library of the chromosomal DNA of a brewing strain (Saccharomyces carlsbergensis) has been made in a Escherichia coli HB101 strain as host. A coloured test allowing one to isolate recombined clones possessing diacetyl (DR) and acetoin (AR) reductase activities is described. This test allowed one to detect 3 Escherichia coli clones having both activities (DR and AR) from among 3000 E. coli strains constituting the genomic library of Saccharomyces carlsbergensis in E. coli. |
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| Keywords: | Diacetyl reductase (DR) acetoin reductase (AR) yeast bacteria coloured reaction |
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