| Abstract: | On the Registration of Endogenous Lipid Peroxidation - in vivo pentane Production in Broiler Chickens This report deals with experimental attempts to clarify the mechanism of pentane development in chicken after feeding fresh and oxidized reesterified triglycerid and different amounts of Vitamin E. In a closed system the concentration of exhaled pentane was determined and in-vivo production rates calculated. On the other hand pentane production was measured from intestinal chyme in an in-vitro preparation after killing the animals. With regard to the question of the microbial cause of pentane development, the effect of Clindamycin was tested both in the in-vitro and in-vivo experiment. In-vivo pentane production rates were increased by feeding oxidized fat, Vitamin E supplementation had no significant influence. Likewise pentane formation from chyme was significantly increased by administering oxidized fats, Vitamin E however inhibited pentane formation. Clindamycin did not suppress pentane production, suggesting that in chicken feed lipid peroxides are not broken down by microbial enzymes but by spontaneous decay, anyway intestinal processes have to be considered in pentane exhalation studies. |
