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琥珀酰化对水酶法提取大豆油的影响
引用本文:李杨,刘雯,江连洲,刘琪,刘珊.琥珀酰化对水酶法提取大豆油的影响[J].中国油脂,2012,37(2):14-18.
作者姓名:李杨  刘雯  江连洲  刘琪  刘珊
作者单位:1. 东北农业大学食品学院,哈尔滨150030;国家大豆工程技术研究中心,哈尔滨150030
2. 东北农业大学食品学院,哈尔滨,150030
摘    要:为了提高大豆水酶法的总油提取率,减少乳状液的形成,采用琥珀酸酐对酶解过程中的水解液进行酰化改性。研究了加酶量、液料比、琥珀酸酐添加量、酶解时间和改性时间对总油提取率的影响,并利用响应面分析优化出最佳工艺参数为:加酶量5 660 U/g,液料比6.34∶1,琥珀酸酐添加量2.95%,酶解时间2.53 h,改性时间2.48 h。在该条件下,总油提取率为(94.49±0.98)%。

关 键 词:琥珀酰化  改性  大豆  水酶法

Effect of succinylation on enzyme- assisted aqueous extraction of soybean oil
LI Yang , LIU Wen , JIANG Lianzhou , LIU Qi , LIU Shan.Effect of succinylation on enzyme- assisted aqueous extraction of soybean oil[J].China Oils and Fats,2012,37(2):14-18.
Authors:LI Yang  LIU Wen  JIANG Lianzhou  LIU Qi  LIU Shan
Affiliation:1(1.Food Science College,Northeast Agricultural University,Harbin 150030,China;2.National Soybean Engineering Technology Research Center,Harbin 150030,China)
Abstract:In order to improve the total oil extraction rate of soybean and reduce the amount of emulsion formed during the enzyme-assisted aqueous extraction,succinic anhydride was used to modify the hydrolysate in the enzymatic hydrolysis process.The influence of the amount of the enzyme,liquid to material ratio,amount of succinic anhydride,hydrolysis time and modification time on the total oil extraction rate was studied.Response surface methodology was employed to optimize the parameters.The total oil extraction rate was(94.49±0.98)% when the optimum parameters were chosen as follows: enzyme amount 5 660 U/g,liquid to material ratio 6.34∶1,amount of succinic anhydride 2.95%,hydrolysis time 2.53 h and modification time 2.48 h.
Keywords:succinylation  modification  soybean  enzyme-assisted aqueous extraction
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