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胰脂肪酶催化樟树籽仁油和甘油合成中碳链单甘油酯
引用本文:曾哲灵,高道龙,龙俊敏,王慧林,邹强,孔令圆,GONG Deming.胰脂肪酶催化樟树籽仁油和甘油合成中碳链单甘油酯[J].中国油脂,2012,37(1):23-28.
作者姓名:曾哲灵  高道龙  龙俊敏  王慧林  邹强  孔令圆  GONG Deming
作者单位:1. 南昌大学食品科学与技术国家重点实验室,南昌330047;南昌大学环境与化学工程学院,南昌330031
2. 南昌大学食品科学与技术国家重点实验室,南昌330047;南昌大学生命科学与食品工程学院,南昌330031
3. 江西生物科技职业学院,南昌,330200
4. 南昌大学生命科学与食品工程学院,南昌,330031
5. 南昌大学食品科学与技术国家重点实验室,南昌,330047
基金项目:科技型中小企业技术创新基金,食品科学与技术国家重点实验室开放基金
摘    要:采用胰脂肪酶催化樟树籽仁油与甘油反应合成中碳链单甘油酯,通过单因素试验和正交试验,研究了反应时间、加酶量、醇油摩尔比、反应初始加水量、反应温度对产物中中碳链单甘油酯含量的影响。结果表明,胰脂肪酶催化樟树籽仁油(10 mL)与甘油反应合成中碳链单甘油酯的适宜条件为:反应温度47℃,加酶量220 mg,醇油摩尔比4.3∶1,反应初始加水量35μL,反应时间30 h。在此反应条件下,产物中中碳链单甘油酯的含量为53.42%±0.05%。

关 键 词:樟树籽仁油  甘油  中碳链单甘油酯  胰脂肪酶  催化  合成

Synthesis of medium-chain monoglycerides from camphor tree seed kernel oil and glycerol catalyzed by Iipase from porcine pancreas
ZENG Zheling , GAO Daolong , LONG Junmin , WANG Huilin , ZOU Qiang , KONG Lingyuan , GONG Deming.Synthesis of medium-chain monoglycerides from camphor tree seed kernel oil and glycerol catalyzed by Iipase from porcine pancreas[J].China Oils and Fats,2012,37(1):23-28.
Authors:ZENG Zheling  GAO Daolong  LONG Junmin  WANG Huilin  ZOU Qiang  KONG Lingyuan  GONG Deming
Affiliation:3,GONG Deming1 (1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;2.School of Environmental and Chemical Engineering,Nanchang University,Nanchang 330031,China;3.School of Life Science and Food Engineering,Nanchang University,Nanchang 330031,China;4.Jiangxi Biotech Vocational College,Nanchang 330200,China)
Abstract:The synthesis technology of medium-chain monoglycerides from camphor tree seed kernel oil and glycerol catalyzed by lipase from porcine pancreas was studied.The effect of reaction time,enzyme dosage,molar ratio of glycerol to camphor tree seed kernel oil,initial water dosage and reaction temperature on the yield of medium-chain monoglycerides was investigated.The optimal synthesis conditions were as follows:oil dosage 10 mL,reaction temperature 47℃,enzyme dosage 220 mg,molar ratio of glycerol to camphor tree seed kernel oil 4.3∶1,initial water dosage 35 μL,and reaction time 30 h.Under the optimal conditions,the content of medium-chain monoglycerides in product reached 53.42%±0.05%.
Keywords:camphor tree seed kernel oil  glycerol  medium-chain monoglycerides  lipase from porcine pancreas  catalysis  synthesis
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