液相色谱-串联质谱法测定植物油中的6种儿茶素

建立液相色谱-串联质谱(LC-MS/MS)测定各种植物油中儿茶素的方法。植物油经水或甲醇液-液萃取,采用ZorbaxSBC18柱,以乙腈–0.1%甲酸为流动相进行梯度洗脱,用多反应监测扫描方式对表没食子儿茶素、儿茶素、表儿茶素、表没食子儿茶素没食子酸酯和没食子儿茶素没食子酸酯等6种儿茶素类茶多酚进行检测。方法的最低检出限均为5~20pg,平均回收率为79.6~99.9%,精密度为0.97%~5.35%。用所建立的方法测定市售植物油中儿茶素类茶多酚的含量。

Determination of 6 catechins in vegetable oils by liquid chromatography-tandem mass spectrometry

A method was developed for the determination of 6 catechins in vegetable oils by liquid chromatography-tandem mass spectrometry(LC-MS/MS).After liquid-liquid extraction using methanol and water,the vegetable oils were chromatographed on a Zorbax SB C18 column with a gradient system of acetonitrile and 0.1% formic acid as mobile phase,then detected using a MS/MS system with electrospray ionization (ESI) in multi-reaction monitoring (MRM) mode. The detection limits of epigallo-catechin, catechin,epicatechin,epigalloc atechin gallate,gallocatechin gallate and epicatechin gallate ranged from 5pg to 20pg. The recoveries ranged from 79.6% to 99.9%, and the precisions (measured as relative standard deviations ) were 0.97%~5.35%. The method has been successfully applied in the determination of catechins in the vegetable oils.