| Abstract: | Facile green synthesis of silver nanoparticles (AgNPs) using an aqueous extract of Carissa carandas (C. carandas) leaves was studied. Fabrication of AgNPs was confirmed by the UV–visible spectroscopy which gives absorption maxima at 420 nm. C. carandas leaves are the rich source of the bioactive molecules, acts as a reducing and stabilising agent in AgNPs, confirmed by Fourier transforms infrared spectroscopy. The field emission scanning electron microscope revealed the spherical shape of biosynthesised AgNPs. A distinctive peak of silver at 3 keV was determined by energy dispersive X‐ray spectroscopy. X‐ray diffraction showed the facecentred cubic structure of biosynthesised AgNPs and thermal stability was confirmed by the thermogravimetric analysis. Total flavonoid and total phenolic contents were evaluated in biosynthesised AgNPs. Biosynthesised AgNPs showed free radical scavenging activities against 2, 2‐diphenyl‐1‐picrylhydrazyl test and ferric reducing antioxidant power assay. In vitro cytotoxicity against hepatic cell lines (HUH‐7) and renal cell lines (HEK‐293) were also assessed. Finally, biosynthesised AgNPs were scrutinised for their antibacterial activity against methicillin‐resistant Staphylococcus aureus, Shigella sonnei, Shigella boydii and Salmonella typhimurium. This study demonstrated the biofabrication of AgNPs by using C. carandas leaves extract and a potential in vitro biological application as antioxidant, anticancer and antibacterial agents.Inspec keywords: antibacterial activity, biomedical materials, cancer, tumours, nanomedicine, silver, nanoparticles, reduction (chemical), nanofabrication, ultraviolet spectra, visible spectra, field emission scanning electron microscopy, Fourier transform infrared spectra, X‐ray chemical analysis, X‐ray diffraction, thermal stability, thermal analysis, free radical reactions, toxicology, cellular biophysics, microorganismsOther keywords: total phenolic contents, free radical scavenging activities, 2,2‐diphenyl‐1‐picrylhydrazyl test, ferric reducing antioxidant power assay, in vitro cytotoxicity, hepatic cell lines HUH‐7, renal cell lines HEK‐293, antibacterial activity, methicillin‐resistant Staphylococcus aureus, Shigella sonnei, Shigella boydii, Salmonella typhimurium, biofabrication, in vitro biological application, Ag, total flavonoid contents, thermogravimetric analysis, thermal stability, face‐centred cubic structure, X‐ray diffraction, energy dispersive X‐ray spectroscopy, distinctive peak, spherical shape, field emission scanning electron microscope, Fourier transforms infrared spectroscopy, stabilising agent, reducing agent, bioactive molecules, absorption maxima, UV‐visible spectroscopy, plant extract colour, antibacterial activities, anticancer activities, antioxidant activities, Carissa carandas, aqueous leaves extract, silver nanoparticles, structural characterisation, one‐pot green synthesis |
