Biogenic synthesis of biopolymer‐based Ag–Au bimetallic nanoparticle constructs and their anti‐proliferative assessment

This study reports an eco‐friendly‐based method for the preparation of biopolymer Ag–Au nanoparticles (NPs) by using gum kondagogu (GK; Cochlospermum gossypium), as both reducing and protecting agent. The formation of GK‐(Ag–Au) NPs was confirmed by UV‐absorption, fourier transformed infrared (FTIR), atomic force microscopy (AFM), scanning electron microscope (SEM) and transmission electron microscope (TEM). The GK‐(Ag–Au) NPs were of 1–12 nm in size. The anti‐proliferative activity of nanoparticle constructs was assessed by MTT assay, confocal microscopy, flow cytometry and quantitative real‐time polymerase chain reaction (PCR) techniques. Expression studies revealed up‐regulation of p53, caspase‐3, caspase‐9, peroxisome proliferator‐activated receptors (PPAR) PPARa and PPARb, genes and down‐regulation of Bcl‐2 and Bcl‐x(K) genes, in B16F10 cells treated with GK‐(Ag–Au) NPs confirming the anti‐proliferative properties of the nanoparticles.Inspec keywords: nanomedicine, transmission electron microscopy, genetics, cellular biophysics, molecular biophysics, enzymes, nanofabrication, gold, silver, scanning electron microscopy, nanoparticles, Fourier transform infrared spectra, atomic force microscopy, biomedical materialsOther keywords: size 1.0 nm to 12.0 nm, Ag‐Au, anti‐proliferative assessment, eco‐friendly‐based method, anti‐proliferative activity, anti‐proliferative properties, biopolymer‐based Ag–Au bimetallic nanoparticle, Cochlospermum gossypium, gum kondagogu, biopolymer preparation, biogenic synthesis, UV‐absorption, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, atomic force microscopy, MTT assay, confocal microscopy, flow cytometry, caspase‐3, caspase‐9, peroxisome proliferator‐activated receptors, Bcl‐2 gene, Bcl‐x(K) gene, B16F10 cells