Silver nanorods induced oxidative stress and chromosomal aberrations in the Allium cepa model |
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| Authors: | Karthiga Durairaj Barsha Roy Natarajan Chandrasekaran Suresh Palamadai Krishnan Amitava Mukherjee |
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| Affiliation: | 1. Centre for Nanobiotechnology, Vellore Institute of Technology, Vellore India ; 2. School of Biosciences and Technology, Vellore Institute of Technology, Vellore India |
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| Abstract: | The production of different size and shape silver nanoparticles (AgNPs) has increased considerably in recent years due to several commercial and biological applications. Here, rod‐shaped AgNPs (SNRs) were prepared using the microwave‐assisted method and characterised by ultraviolet–visible spectroscopy, and transmission electron microscopy analysis. The present study aims to investigate the cyto–genotoxic effect of various concentrations (5, 10, and 15 µM) of SNRs using Allium cepa model. As a result, concentration‐dependent cyto–genotoxic effect of SNRs was observed through a decrease in the mitotic index, and an increase in the chromosomal aberrations such as chromosome break, disturbed metaphase, and anaphase bridge. To check the impact of Ag+ ions, 15 µM silver nitrate (AgNO3) was prepared and tested in all the assays. Furthermore, cell viability and different reactive oxygen species assays were performed to test the cytotoxicity evaluation of SNRs. The authors found that in all the tested assays, SNRs at high concentrations (15 µM) and AgNO3 (15 µM) were observed to cause maximal damage to the roots. Therefore, the current study implies that the cytotoxicity and genotoxicity of SNRs were dependent on the concentration of SNRs.Inspec keywords: ultraviolet spectra, biomedical materials, silver, cellular biophysics, nanorods, toxicology, visible spectra, nanoparticles, nanofabrication, transmission electron microscopy, nanomedicine, aberrations, microwave materials processingOther keywords: biological applications, rod‐shaped AgNPs, microwave‐assisted method, ultraviolet‐visible spectroscopy, transmission electron microscopy, chromosomal aberrations, chromosome break, silver nitrate, reactive oxygen species assays, cytotoxicity, genotoxicity, silver nanorods, oxidative stress, shape silver nanoparticles, concentration‐dependent cyto‐genotoxic effect, Allium cepa model, mitotic index, disturbed metaphase, anaphase bridge, cell viability, cytotoxicity evaluation, Ag |
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