Evaluation of the toxicities of silver and silver sulfide nanoparticles against Gram‐positive and Gram‐negative bacteria |
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Authors: | Siva Bala Subramaniyan Sengan Megarajan Santhosh Vijayakumar Mariappan Mariappan Veerappan Anbazhagan |
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Affiliation: | 1. Department of Chemistry, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401 Tamil Nadu, India ; 2. Department of Chemistry, SRM University, Kattankulathur, Chennai Tamil Nadu, India |
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Abstract: | In this study, the endogenous lipid signalling molecules, N ‐myristoylethanolamine, were explored as a capping agent to synthesise stable silver nanoparticles (AgNPs) and Ag sulphide NPs (Ag2 S NPs). Sulphidation of the AgNPs abolishes the surface plasmon resonance (SPR) maximum of AgNPs at 415 nm with concomitant changes in the SPR, indicating the formation of Ag2 S NPs. Transmission electron microscopy revealed that the AgNPs and Ag2 S NPs are spherical in shape with a size of 5–30 and 8–30 nm, respectively. AgNPs and Ag2 S NPs exhibit antimicrobial activity against Gram‐positive and Gram‐negative bacteria. The minimum inhibitory concentrations (MIC) of 25 and 50 μM for AgNPs and Ag2 S NPs, respectively, were determined from resazurin microtitre plate assay. At or above MIC, both AgNPs and Ag2 S NPs decrease the cell viability through the mechanism of membrane damage and generation of excess reactive oxygen species.Inspec keywords: cellular biophysics, biomembranes, transmission electron microscopy, nanomedicine, microorganisms, molecular biophysics, antibacterial activity, nanofabrication, silver, biomedical materials, surface plasmon resonance, nanoparticles, materials preparation, silver compounds, lipid bilayersOther keywords: Gram‐negative bacteria, Gram‐positive bacteria, endogenous lipid signalling molecules, N‐myristoylethanolamine, capping agent, silver nanoparticles, Ag sulphide NPs, sulphidation, surface plasmon resonance, concomitant changes, transmission electron microscopy, minimum inhibitory concentrations, resazurin microtitre plate assay, cell viability, membrane damage, reactive oxygen species, Ag toxicities, Ag, Ag2 S |
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