Salicylic acid nanoparticles (SANPs) improve growth and phytoremediation efficiency of Isatis cappadocica Desv., under As stress

Arsenic (As) is a toxic metalloid dispersed in the environment and it cause serious toxicity to plants. Salicylic acid (SA) plays an important role in many plant growth processes involved in plant defence against heavy metal or metalloid stress. In the present study, for the first time, chitosan nanoparticles was synthesised, loaded by SA and the positive role of SA on growth and phytoremediation efficiency of Isatis cappadocica against As toxicity were evaluated. The highest arsenate treatment (1200 µM) caused a decrease in plant biomass that, however, its combined application with salicylic acid nanoparticles (SANPs) substantially increase in shoot height, root length and their biomass production compared with As stressed plants. The pretreatment of SANPs by increasing arsenate supply, simultaneously increased the As concentration in roots and shoots of I. cappadocica which reached a maximum of 705 and 1188 mg/kg, respectively. This results suggest that high levels of As stress induce stress status in I. cappadocica which SANPs pretreatment application limit these toxic effects of As. Therefore, SANPs has a significant beneficial effect on the growth and phytoremediation efficiency of I. cappadocica subjected to As stress.Inspec keywords: nanoparticles, nanobiotechnology, botany, toxicology, arsenic, biological techniques, polymersOther keywords: salicylic acid nanoparticles, SANP, plant growth efficiency, phytoremediation efficiency, Isatis cappadocica Desv, arsenic stress, toxic metalloid, plant toxicity, plant defence, metalloid stress, chitosan nanoparticles, plant biomass, shoot height, root length, biomass production, I. cappadocica, As