Targeting microbial biofilms: by Arctium lappa l. synthesised biocompatible CeO2 ‐NPs encapsulated in nano‐chitosan |
| |
| Authors: | Bushra Uzair Nousheen Akhtar Shamaila Sajjad Asma Bano Fehmida Fasim Naheed Zafar Sajjad Ahmed Khan Leghari |
| |
| Affiliation: | 1. Department of Biological Sciences, International Islamic University, Islamabad Pakistan ; 2. Department of Physics, International Islamic University, Islamabad Pakistan ; 3. Department of Microbiology, University of Haripur, Haripur Pakistan ; 4. Discipline of Biomedical Science, Sydney Medical School, The University of Sydney, Sydney NSW, Australia ; 5. Pakistan Institute of Engineering and Applied Sciences, Islamabad Pakistan |
| |
| Abstract: | This study is planned to synthesise new biocompatible, nano antimicrobial formulation against biofilm producing strains. Aqueous root extract of Arctium lappa l. was used to synthesise ceria nanoparticles (CeO2 ‐NPs). The synthesised nanoparticles were encapsulated with nano‐chitosan by sol–gel method and characterised using standard techniques. Gas chromatography‐mass spectrometer of Arctium lappa l. revealed the presence of ethanol, acetone, 1‐ propanol, 2‐methylethane, 1,1‐di‐ethoxy, 1‐Butanol, and oleic acid acted as reducing and surface stabilising agents for tailoring morphology of CeO2 ‐NPs. Erythrocyte integrity after treatment with synthesised nanomaterials was evaluated by spectrophotometer measurement of haemoglobin release having biocompatibility. Scanning electron microscopy revealed the formation of mono dispersed beads shaped particles with mean particle size of 26.2 nm. X‐ray diffractometry revealed cubic crystalline structure having size of 28.0 nm. After encapsulation by nano‐chitosan, the size of CeO2 ‐NPs enhances to 48.8 nm making average coverage of about 22.6 nm. The synthesised nanomaterials were found effective to disrupt biofilm of S. aureus and P. aeruginosa. Interestingly, encapsulated CeO2 ‐NPs revealed powerful antibacterial and biofilm disruption activity examined by fluorescent live/dead staining using confocal laser scanning microscopy. The superior antibacterial activities exposed by encapsulated CeO2 ‐NPs lead to the conclusion that they could be useful for controlling biofilm producing multidrug resistance pathogens.Inspec keywords: particle size, microorganisms, organic compounds, nanomedicine, sol‐gel processing, cellular biophysics, scanning electron microscopy, optical microscopy, nanoparticles, antibacterial activity, fluorescence, biomedical materials, nanofabrication, X‐ray diffraction, chromatography, filled polymers, cerium compoundsOther keywords: microbial biofilms, aqueous root extract, sol–gel method, gas chromatography‐mass spectrometer, 1‐di‐ethoxy, 1‐Butanol, nanomaterial synthesis, mean particle size, antibacterial activities, ethanol, acetone, 1‐ propanol, biocompatible ceria‐nanoparticle encapsulation, nano‐chitosan, Arctium lappa l., oleic acid, erythrocyte integrity, spectrophotometer measurement, haemoglobin release, mono dispersed beads shaped particle formation, X‐ray diffractometry, cubic crystalline structure, fluorescent live/dead staining, confocal laser scanning microscopy, multidrug resistance pathogens, size 26.2 nm, size 28.0 nm, size 48.8 nm, size 22.6 nm, CeO2 |
| |
| Keywords: | |
|
|
