Photo‐induced Leishmania DNA degradation by silver‐doped zinc oxide nanoparticle: an in‐vitro approach |
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| Authors: | Akhtar Nadhman Muhammad Sirajuddin Samina Nazir Masoom Yasinzai |
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| Affiliation: | 1. Department of Biotechnology, Quaid‐i‐Azam University, Islamabad Pakistan ; 2. Nanosciences and Catalysis Division, National Centre for physics, Shahdra Valley Road, Quaid‐i‐Azam University campus, Islamabad Pakistan ; 3. Department of Chemistry, University of Science & Technology, Bannu Pakistan ; 4. Center of Interdisciplinary Research, International Islamic University, Islamabad Pakistan |
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| Abstract: | Recently, the authors reported newly synthesised polyethylene glycol (PEG)ylated silver (9%)‐doped zinc oxide nanoparticle (doped semiconductor nanoparticle (DSN)) which has high potency for killing Leishmania tropica by producing reactive oxygen species on exposure to sunlight. The current report is focused on Leishmania DNA interaction and damage caused by the DSN. Here, we showed that the damage to Leishmania DNA was indirect, as the DSN was unable to interact with the DNA in intact Leishmania cell, indicating the incapability of PEGylated DSN to cross the nucleus barrier. The DNA damage was the result of high production of singlet oxygen on exposure to sunlight. The DNA damage was successfully prevented by singlet oxygen scavenger (sodium azide) confirming involvement of the highly energetic singlet oxygen in the DNA degradation process.Inspec keywords: silver, zinc compounds, nanoparticles, nanomedicine, DNA, microorganisms, cellular biophysics, biomedical engineeringOther keywords: photo‐induced Leishmania DNA degradation, PEGylated silver‐doped zinc oxide nanoparticle, Leishmania tropica, reactive oxygen species, sunlight, Leishmania DNA interaction, Leishmania cell, DNA damage, singlet oxygen scavenger, sodium azide, DNA degradation process, ZnO:Ag |
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