Abstract: | In this study, green synthesis of gold nanoparticles (AuNPs) was performed by a sunlight irradiation method using the Borassus flabellifer fruit extract as a reducing agent. 5‐Fluorouracil (5‐FU)‐loaded GG capped AuNPs (5FU‐G‐AuNPs) was prepared. The nanoparticles was further characterised by UV‐visible spectra, particle size analysis, zeta potential, SAED, HRTEM, and XRD. The MTT assay results showed the suitability 5‐FU‐G‐AuNPs. In this study, 5‐FU‐G‐AuNPs exhibited potential cytotoxic and apoptotic effects on (MiaPaCa‐2) cell line.Inspec keywords: gold, biochemistry, X‐ray diffraction, nanofabrication, biomedical materials, transmission electron microscopy, toxicology, electrokinetic effects, particle size, nanoparticles, cancer, visible spectra, cellular biophysics, ultraviolet spectra, nanomedicine, patient treatment, organic compoundsOther keywords: 5FU‐G‐AuNPs, suitability 5‐FU‐G‐AuNPs, human pancreatic cancer cell, green synthesis, sunlight irradiation method, 5‐Fluorouracil‐loaded GG, in vitro treatment, 5 fluorouracil‐loaded biosynthesised gold nanoparticles, borassus flabellifer fruit extract, reducing agent, UV‐visible spectra, particle size analysis, zeta potential, SAED, HRTEM, XRD, MTT assay, apoptotic effects, cytotoxic effects, MiaPaCa‐2 cell line, Au |