Antifungal and antiovarian cancer properties of α Fe2 O3 and α Fe2 O3 /ZnO nanostructures synthesised by Spirulina platensis
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| Authors: | Heba Salah Abbas Akilandeswari Krishnan Muddukrishnaiah Kotakonda |
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| Affiliation: | 1. National Organization for Drug Control and Research, Cairo Egypt ; 2. Scientist, under scheme of Asian Research Training Fellowship for Developing Country (RTF‐DCS), FICCI, New Delhi India ; 3. Department of Pharmaceutical Technology, Bharathidasan Institute of Technology, Anna University, Tiruchirappalli 620024 Tamilnadu, India |
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| Abstract: | Candida albicans (C. albicans) infection shows a growing burden on human health, and it has become challenging to search for treatment. Therefore, this work focused on the antifungal activity, and cytotoxic effect of biosynthesised nanostructures on human ovarian tetracarcinoma cells PA1 and their corresponding mechanism of cell death. Herein, the authors fabricated advanced biosynthesis of uncoated α‐Fe2 O3 and coated α‐Fe2 O3 nanostructures by using the carbohydrate of Spirulina platensis. The physicochemical features of nanostructures were characterised by UV–visible, high resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and X‐ray diffraction. The antifungal activity of these nanostructures against C. albicans was studied by the broth dilution method, and examined by 2′, 7′‐dichlorofluorescein diacetate staining. However, their cytotoxic effects against PA1 cell lines were evaluated by MTT and comet assays. Results indicated characteristic rod‐shaped nanostructures, and increasing the average size of α‐Fe2 O3 @ZnO nanocomposite (105.2 nm × 29.1 nm) to five times as compared to α‐Fe2 O3 nanoparticles (20.73nm × 5.25 nm). The surface coating of α‐Fe2 O3 by ZnO has increased its antifungal efficiency against C. albicans. Moreover, the MTT results revealed that α‐Fe2 O3 @ZnO nanocomposite reduces PA1 cell proliferation due to DNA fragmentation (IC50 18.5 μg/ml). Continual advances of green nanotechnology and promising findings of this study are in favour of using the construction of rod‐shaped nanostructures for therapeutic applications.Inspec keywords: nanocomposites, toxicology, nanofabrication, cellular biophysics, X‐ray diffraction, iron compounds, biochemistry, cancer, antibacterial activity, transmission electron microscopy, biomedical materials, wide band gap semiconductors, DNA, II‐VI semiconductors, visible spectra, molecular biophysics, ultraviolet spectra, nanomedicine, zinc compounds, nanoparticles, microorganisms, Fourier transform infrared spectraOther keywords: Spirulina platensis, antifungal activity, α‐Fe2 O3 nanoparticles, antiovarian cancer properties, Candida albicans infection, cytotoxic effect, biosynthesised nanostructures, human ovarian tetracarcinoma cell PA1, cell death, uncoated α‐Fe2 O3 , coated α‐Fe2 O3 nanostructures, α‐Fe2 O3 ‐ZnO nanocomposite, carbohydrate, physicochemical features, UV‐visible spectroscopy, high resolution transmission electron microscopy, Fourier transform infrared spectroscopy, X‐ray diffraction, broth dilution method, 2′, 7′‐dichlorofluorescein diacetate staining, PA1 cell lines, comet assays, MTT assays, rod‐shaped nanostructures, surface coating, PA1 cell proliferation, DNA fragmentation, green nanotechnology, Fe2 O3 ‐ZnO, Fe2 O3 |
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