Hepatic histopathological and ultrastructural alterations induced by 10 nm silver nanoparticles

Silver nanoparticles (Ag NPs) are invested in various sectors and are becoming more persistent in our ambient environment with potential risk on our health and the ecosystems. The current study aims to investigate the histological, histochemical and ultrastructural hepatic changes that might be induced by 10 nm silver nanomaterials. Male mice (BALB/C) were exposed for 35 injections of daily dose of 10 nm Ag NPs (2 mg/kg). Liver tissues were subjected to examination by light and electron microscopy for histological, histochemical and ultrastructural alterations. Exposure to Ag NPs induced Kupffer cells hyperplasia, sinusoidal dilatation, apoptosis, ground glass hepatocytes appearance, nuclear changes, inflammatory cells infiltration, hepatocytes degeneration and necrosis. In addition, 10 nm Ag NPs induced histochemical alterations mainly glycogen depletion with no hemosiderin precipitation. Moreover, these nanomaterials exhibited ultrastructure alterations including mitochondrial swelling and cristolysis, cytoplasmic vacuolation, apoptosis, multilammellar myelin figures formation and endoplasmic destruction and reduction. The findings revealed that Ag NPs can induce alterations in the hepatic tissues, the chemical components of the hepatocytes and in the ultrastructure of the liver. One may also conclude that small size Ag NPs, which are increasingly used in human products could cause various toxigenic responses to all hepatic tissue components.Inspec keywords: liver, electron microscopy, molecular biophysics, optical microscopy, toxicology, biochemistry, silver, biological tissues, nanofabrication, cellular biophysics, biomedical materials, nanoparticles, nanomedicineOther keywords: hepatic histopathological alterations, ultrastructural alterations, silver nanoparticles, histological changes, histochemical changes, ultrastructural hepatic changes, silver nanomaterials, male mice, liver tissues, electron microscopy, histological alterations, histochemical alterations, Kupffer cells hyperplasia, sinusoidal dilatation, apoptosis, ground glass hepatocytes appearance, inflammatory cells infiltration, hepatocyte degeneration, necrosis, ultrastructure alterations, Ag, size 10.0 nm, hepatic tissue components, cytoplasmic vacuolation