Comparative antibacterial activity of silver nanoparticles synthesised by biological and chemical routes with pluronic F68 as a stabilising agent |
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| Authors: | Carolina Alves dos Santos Marcelo Martins Seckler Avinash P Ingle Mahendra Rai |
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| Affiliation: | 1. Department of Chemical Engineering, Polytechnic School, University of Sao Paulo, Sao Paulo, Brazil ; 2. Laboratory of Biomaterials and Nanotechnology, University of Sorocaba. i(bs)2‐Intelligent Biosensing and Biomolecule Stabilization Research Group, Sorocaba SP, Brazil ; 3. Nanobiotechnology Laboratory, Department of Biotechnology, SGB Amravati University, Amravati 444 602 Maharashtra, India |
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| Abstract: | The authors report the comparative antibacterial activity of silver nanoparticles synthesised by biological (using Fusarium oxysporum) and chemical routes in the presence and absence of pluronic F68 as a stabilising agent. The production of silver nanoparticles was evidenced by UV–visible spectra, with absorbance at about 420 nm in the case of both biological and chemical synthesis. X‐ray diffraction pattern confirmed the presence of face‐centred cubic structure (FCC plane). The nanoparticles characterised by transmission and scanning electron microscopy showed spherical silver nanoparticles with size range of 5–40 and 10–70 nm in the case of biologically and chemically synthesised nanoparticles, respectively. Addition of pluronic F68 showed the stabilisation of silver nanoparticles. Antibacterial efficacy of silver nanoparticles demonstrated different inhibitory activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Overall, biologically synthesised silver nanoparticles showed higher activity as compared with chemically synthesised nanoparticles. Silver nanoparticles synthesised in the presence of pluronic F68 by the chemical route exhibited synergism in antibacterial activity as compared with those synthesised without pluronic F68. On the contrary, biogenic silver nanoparticles without pluronic F68 showed higher antibacterial potential.Inspec keywords: antibacterial activity, nanofabrication, silver, X‐ray diffraction, biomedical materials, nanomedicine, transmission electron microscopy, scanning electron microscopy, ultraviolet spectra, visible spectra, materials preparation, nanoparticlesOther keywords: pluronic F68, stabilising agent, comparative antibacterial activity, Fusarium oxysporum, UV‐visible spectra, biological synthesis, chemical synthesis, X‐ray diffraction pattern, face‐centred cubic structure, FCC plane, transmission electron microscopy, scanning electron microscopy, spherical silver nanoparticles, antibacterial efficacy, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, synergism, biogenic silver nanoparticles, wavelength 420 nm, size 10 nm to 70 nm, size 5 nm to 40 nm, Ag |
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