In Vitro Framework to Assess the Anti-Helicobacter pylori Potential of Lactic Acid Bacteria Secretions as Alternatives to Antibiotics |
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| Authors: | Samantha A Whiteside Mahi M Mohiuddin Sargon Shlimon Jaspreet Chahal Chad W MacPherson Jana Jass Thomas A Tompkins Carole Creuzenet |
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| Affiliation: | 1.Infectious Diseases Research Group, Department of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5C1, Canada; (S.A.W.); (M.M.M.); (S.S.); (J.C.); (J.J.);2.Lallemand Health Solutions, Montreal, QC H4P 2R2, Canada; (C.W.M.); (T.A.T.);3.The Canadian Research and Development Centre for Probiotics, London Health Research Institute, London, ON N6A 4V2, Canada |
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| Abstract: | Helicobacter pylori is a prevalent bacterium that can cause gastric ulcers and cancers. Lactic acid bacteria (LAB) ameliorate treatment outcomes against H. pylori, suggesting that they could be a source of bioactive molecules usable as alternatives to current antibiotics for which resistance is mounting. We developed an in vitro framework to compare the anti-H. pylori properties of 25 LAB and their secretions against H. pylori. All studies were done at acidic and neutralized pH, with or without urea to mimic various gastric compartments. Eighteen LAB strains secreted molecules that curtailed the growth of H. pylori and the activity was urea-resistant in five LAB. Several LAB supernatants also reduced the urease activity of H. pylori. Pre-treatment of H. pylori with acidic LAB supernatants abrogated its flagella-mediated motility and decreased its ability to elicit pro-inflammatory IL-8 cytokine from human gastric cells, without reverting the H. pylori-induced repression of other pro-inflammatory cytokines. This study identified the LAB that have the most anti-H. pylori effects, decreasing its viability, its production of virulence factors, its motility and/or its ability to elicit pro-inflammatory IL-8 from gastric cells. Once identified, these molecules can be used as alternatives or complements to current antibiotics to fight H. pylori infections. |
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| Keywords: | Helicobacter pylori lactic acid bacteria secretome urease inflammation cytokines gastric disease |
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