响应曲面法优化大蒜中总酚提取工艺及其抗氧化活性测定

采用响应曲面法对料液比、提取时间、不同体积分数酸化甲醇提取剂3个因素进行优化,以料液比、提取时间、不同体积分数酸化甲醇提取剂为自变量,总酚提取量为响应值,利用Box-Behnken设计原理和响应曲面法,研究各自变量及其交互作用对总酚提取的影响,模拟求得二次多项回归方程的预测模型,并确定料液比1:14(g/mL)、提取时间31min、80%酸化甲醇为提取剂是最佳的提取条件。在此条件下,总酚提取量为0.67mg GAE/g,模型预测值偏差为6.9%,证明所选工艺条件为最佳工艺条件。同时,用5种方法对其抗氧化活性进行测定,结果表明大蒜具有良好的抗氧化活性。

Response Surface Methodology for Optimization of Extraction Conditions of Total Phenolic Compounds from Garlic and Determination of Antioxidant Activity

Response Surface Methodology(RSM) was used to optimize extraction parameters(including material/liquid ratio,extraction time and concentration of extracting agents) so as to achieve the maximum extraction yield of total phenolic compounds.Studies were carried out on the respective independent variables(material/liquid ratio,extraction time and concentration of extracting agents) and the interactive effects of these three factors on the total phenolic compounds according to Box-Benhnken design.A mathematical quadratic polynomial regression equation reflecting the relationship between the total phenolic compounds and the above extraction parameters was established.The optimal extraction parameters were obtained as: material/ liquid ratio 1:14(g/mL),extraction time 31 min,and 80% acidification methanol as extracting agent.Under the optimal condition,the total phenolic content was 0.67 mg GAE/g,and the model prediction deviation was 6.9%.Meanwhile,the antioxidant activity was determined with five different methods,and the results showed that garlic had good antioxidant activity.