Auxiliary method for clonal identification of Staphylococcus aureus by protein band pattern of released proteins on SDS-polyacrylamide gel |
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| Authors: | K Seki J Sakurada M Murai A Usui HK Seong H Jitsukawa S Masuda |
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| Affiliation: | Department of Bacteriology, Jikei University School of Medicine, Tokyo, Japan. |
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| Abstract: | A supportive method for clonal identification of Staphylococcus aureus strains was devised. Culture supernatant obtained by cellophane surface culture was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) without performing any concentration procedure prior to electrophoresis. The combined use of cellophane surface culture and SDS-PAGE was convenient for determining whether the strains belonged to the same clone or not when conducted in conjunction with other tests for bacteriological characterization. |
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