Quantification of metabolites from single-voxelin vivo
1H NMR data of normal human brain by means of time-domain data analysis |
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| Authors: | Mika Ala-Korpela Jussi-Pekka Usenius Jyrki Keisala Aad van den Boogaart Pauli Vainio Jukka Jokisaari Seppo Soimakallio Risto Kauppinen |
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| Affiliation: | (1) Department of Physical Sciences, NMR Research Group University of Oulu, Oulu, Finland;(2) Hammersmith Hospital, MR Unit, London, UK;(3) Department of Clinical Radiology, University Hospital of Kuopio, Kuopio, Finland;(4) Division of Biochemistry, CRC Biomedical Magnetic Resonance Research Group, St. George's Hospital Medical School, London, UK;(5) NMR Research Group, Department of Biochemistry and Biotechnology, A.I. Virtanen Institute, University of Kuopio, FIN-70211 Kuopio, Finland |
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| Abstract: | We present here a combination of time-domain signal analysis procedures for quantification of human brainin vivo
1H NMR spectroscopy (MRS) data. The method is based on a separate removal of a residual water resonance followed by a frequency-selective time-domain line-shape fitting analysis of metabolite signals. Calculation of absolute metabolite concentrations was based on the internal water concentration as a reference. The estimated average metabolite concentrations acquired from six regions of normal human brain with a single-voxel spin-echo technique for theN-acetylaspartate, creatine, and choline-containing compounds were 11.4±1.0,6.5±0.5, and 1.7±0.2 mmol kg–1 wet weight, respectively. The time-domain analyses ofin vivo
1H MRS data from different brain regions with their specific characteristics demonstrate a case in which the use of frequency-domain methods pose serious difficulties. |
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| Keywords: | brain metabolites 1H NMR spectroscopy quantification |
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