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A universal expression tag for structural and functional studies of proteins |
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| Authors: | Rogov Vladimir V Rozenknop Alexis Rogova Natalia Yu Löhr Frank Tikole Suhas Jaravine Victor Güntert Peter Dikic Ivan Dötsch Volker |
| Affiliation: | Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Strasse 9, 60438 Frankfurt, Germany. rogov@bpc.uni-frankfurt.de |
| Abstract: | Modified ubiquitin sequences, each completed with a His tag and a TEV cleavage site, were designed to enhance the expression of protein/peptide targets. With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers. |
| Keywords: | NMR spectroscopy protein expression solubility tags ubiquitin |
| 本文献已被 PubMed 等数据库收录！ | |