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Chromatographic measurement of urinary steroids in patients with psoriasis
Authors:VP Logunov  SA Mazkhar
Abstract:A gas-chromatographic method for urinary steroid measurements by Chrome-5 (Prague) chromatograph with plasma ionization detector is suggested. Glass column 1.5 m long is packed with N-AW chromatone impregnated with 15% SE-30. Detector and evaporator temperature is 260 degrees C. Column temperature is programmed from 200 to 250 degrees C. Quantitative and qualitative analysis of chromatograms in 40 patients and 30 controls permitted assessment of excretion of 17-ketosteroids, hydroxyandrosterone, etiocholanolone, androsterone, dihydroepiandrosterone, beta-cortol compounds, etc., which is important in studies of psoriasis pathogenesis and in assessment of the efficacy of treatment of this and other diseases.
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