Influence of genotype on diet-induced changes in membrane phosphatidylcholine and phosphatidylethanolamine composition of splenocytes,liver nuclear envelope and liver mitochondria |
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Authors: | J T Venkatraman R K Tiwari B Cinader J Flory T Wierzbicki M T Clandinin |
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Affiliation: | (1) Nutrition and Metabolism Research Group, Departments of Foods & Nutrition and Medicine, Division of Gastroenterology, University of Alberta, T6G 2C2 Edmonton, Alberta, Canada;(2) Present address: Sloan Kettering Institute for Cancer Research, New York, New York;(3) Present address: Department of Immunology, University of Toronto, Ontario, Canada;(4) Department of Foods & Nutrition, University of Alberta, 318f Home Economics Building, T6G 2M8 Edmonton, Alberta, Canada |
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Abstract: | Inbred congenic mice of strains MRL/Mp-lpr/lpr (lpr/lpr) and MRL/Mp-+/+ (+/+) were fed nutritionally adequate semipurified
diets containing 20% (w/w) fat and differing in linoleic acid content. Levels of linoleic acid (18∶2n−6) and arachidonic acid
(20∶4n−6) in phospholipids of splenocytes, liver mitochondria and liver nuclear envelopes were determined. Membranes of lpr/lpr
mice exhibited significantly lower levels of 18∶2n−6 and 20∶4n−6 in phospholipids compared with the +/+ strain. The high linoleic
acid diet increased incorporation of 18∶2n−6 and 20∶4n−6 in most phospholipid fractions of these membranes. These observations
indicate that genotype as well as dietary 18∶2n−6 content significantly influenced incorporation of 18∶2n−6 and 20∶4n−6 into
membrane phospholipids. The results also suggest that membrane compositional abnormalities found in the lpr/lpr mice, which
develop lymphoma and age faster than +/+ mice, are not restricted to the immune system but also extend to other organs. Differences
observed in phospholipid fatty acid composition in splenocytes and liver subcellular membranes for mice fed diets differing
in linoleic acid content suggest that the early expression of the lpr gene resulting in progression of autoimmunity may be
delayed through dietary manipulation. |
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