Bovine Milk Formula Based on Partial Hydrolysis of Caseins by Bromelain Enzyme: Better Digestibility and Angiotensin-Converting Enzyme-Inhibitory Properties |
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| Authors: | Vera Medeiros Nuno Rainha Lisete Paiva Elisabete Lima |
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| Affiliation: | 1. UNILEITE, Largo das Arribanas, Arrifes , Ponta Delgada , Azores , Portugal;2. Department of Technological Sciences and Development and Investigation Center on Natural Resources , University of Azores , Ponta Delgada , Azores , Portugal |
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| Abstract: | A new methodology was developed using bromelain as a proteolytic enzyme for the production of a partially hydrolyzed bovine milk formula, without changing the milk's organoleptic and nutritional properties. This study also revealed that the in vitro angiotensin-converting enzyme inhibition increased in the modified milk as compared with the control sample, showing the highest angiotensin-converting enzyme-inhibitory activity for fractions with molecular weights between 3 and 10 kg mol?1. The Lineweaver-Burk plot revealed that the produced milk formula acted as a non-competitive inhibitor, as determined by high performance liquid chromatography. |
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| Keywords: | ACE-inhibitory activity Bromelain Milk protein allergy Partially hydrolyzed bovine milk formula Physicochemical and microbiological properties RP-HPLC/UV Sensory analysis |
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