An aptamer-based microfluidic device for thermally controlled affinity extraction |
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Authors: | ThaiHuu Nguyen Renjun Pei Milan Stojanovic Qiao Lin |
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Affiliation: | (1) Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA;(2) Department of Medicine, Columbia University, New York, NY 10032, USA |
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Abstract: | We present a microfluidic device for specific extraction and thermally activated release of analytes using nucleic acid aptamers. The device primarily consists of a microchamber that is packed with aptamer-functionalized microbeads as a stationary phase, and integrated with a micro heater and temperature sensor. We demonstrate the device operation by performing the extraction of a metabolic analyte, adenosine monophosphate coupled with thiazole orange (TO-AMP), with high selectivity to an RNA aptamer. Controlled release of TO-AMP from the aptamer surface is then conducted at low temperatures using on-chip thermal activation. This allows isocratic analyte elution, which eliminates the use of potentially harsh reagents, and enables efficient regeneration of the aptamer surfaces when device reusability is desired. |
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Keywords: | Affinity binding Isocratic elution Microfluidics Purification Solid-phase extraction Specificity Thermal release |
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