不同提取条件对苦荞粉中芦丁提取率的影响

研究了不同提取条件对苦养粉中芦丁提取率及芦丁降解的影响,设计正交试验,优化芦丁提取工艺条件,优化提取条件为:体积分数为50%的乙醇溶液,料液比(g:mL)1:10,60℃条件下提取1h,提取2次。采用薄层层析法(TLC),分析了低体积分数乙醇溶液中,芦丁在芦丁降解酶的作用下降解,有乙基-β-芸香糖苷的生成。因此,为保证芦丁的高效提取,乙醇体积分数应高于50%。

Effect of Different Extraction Conditions on Rutin of Tartary Buckwheat Flour

The effect of different extraction conditions on rutin of tartary buckwheat flour and the degra- dation reaction of rutin were studied.The optimum conditions on rutin were obtained through orthogonal de- sign.The optimum conditions is ten times volumes,50% alcohlol-water,temperature 60℃,extracting 1h and twice times.Degradation products of rutin were analyzed by TLC.Rutin was degraded under the action of rutin hydrolase(β-glycosidase)and ethyl-β-rutinose was produced in alcohol solution.The result show that ethanol concentration should be above 50% to ensure the effective extraction of rutin.